Oligonucleotide Synthesis
Author | : Piet Herdewijn |
Publisher | : Springer Science & Business Media |
Total Pages | : 436 |
Release | : 2008-02-04 |
ISBN-10 | : 9781592598236 |
ISBN-13 | : 1592598234 |
Rating | : 4/5 (234 Downloads) |
Download or read book Oligonucleotide Synthesis written by Piet Herdewijn and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.