Download or read book Bridging Chromatin Architecture to Gene Expression written by Jennifer Crutchley and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "There has been a great influx of information regarding the structures that our genome is packaged into, but how this occurs and how it relates to gene expression remains a mystery. Recently, Hi-C technology has revealed that our genome is organized in topological associated domains (TADs) and that at the boundaries of these TADs there is an increase of the previously characterized chromatin organization mediators, CTCF and cohesin. It is my objective to identify the role of CTCF and cohesin in the regulation of the gene expression and TAD boundary maintenance of the HOXA cluster. By employing siRNA and shRNA methodology in combination of 5C-seq technology, I identified that cohesin is the key mediator involved in maintaining the TAD boundary within the HOXA locus. Furthermore, loss of the cohesin complex resulted in an up-regulation of the 5'end HOXA genes, suggesting that the maintenance boundary is required to shut down the expression of the genes within that region. The results suggest that cohesin is the key mediator involved in the regulation and higher order chromatin structure of the HOXA cluster." --