E. coli Plasmid Vectors

E. coli Plasmid Vectors
Author :
Publisher : Springer Science & Business Media
Total Pages : 311
Release :
ISBN-10 : 9781592594092
ISBN-13 : 1592594093
Rating : 4/5 (093 Downloads)

Book Synopsis E. coli Plasmid Vectors by : Nicola Casali

Download or read book E. coli Plasmid Vectors written by Nicola Casali and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.


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