PCR Mutation Detection Protocols

PCR Mutation Detection Protocols
Author :
Publisher : Springer
Total Pages : 224
Release :
ISBN-10 : 1617371009
ISBN-13 : 9781617371004
Rating : 4/5 (004 Downloads)

Book Synopsis PCR Mutation Detection Protocols by : Bimal D. M. Theophilus

Download or read book PCR Mutation Detection Protocols written by Bimal D. M. Theophilus and published by Springer. This book was released on 2010-10-28 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.


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