Enzyme-Mediated Immunoassay

Enzyme-Mediated Immunoassay
Author :
Publisher : Springer Science & Business Media
Total Pages : 481
Release :
ISBN-10 : 9781468450125
ISBN-13 : 1468450123
Rating : 4/5 (123 Downloads)

Book Synopsis Enzyme-Mediated Immunoassay by : H.M. Lenhoff

Download or read book Enzyme-Mediated Immunoassay written by H.M. Lenhoff and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA). Twelve years later, Engvall and Perlmann (1971) and Van Weemen and Schuurs (1971) independently introduced the use of enzymes as another category of sensitive and even more versatile labels for use in immunoassays. Engvall and Perlmann (l971) coined the term ELISA, which stands for Enzyme Linked Immunosorbent Assay.


Enzyme-Mediated Immunoassay Related Books

Enzyme-Mediated Immunoassay
Language: en
Pages: 481
Authors: H.M. Lenhoff
Categories: Science
Type: BOOK - Published: 2012-12-06 - Publisher: Springer Science & Business Media

GET EBOOK

T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin label
Enzyme-linked Immunosorbent Assay (ELISA)
Language: en
Pages: 124
Authors: Samira Hosseini
Categories: Technology & Engineering
Type: BOOK - Published: 2017-12-30 - Publisher: Springer

GET EBOOK

This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytic
Enzyme Immunoassay
Language: en
Pages: 471
Authors: Edward T. Maggio
Categories: Health & Fitness
Type: BOOK - Published: 2018-01-18 - Publisher: CRC Press

GET EBOOK

The purpose of this book is to focus attention on some of these ideas and concepts. In doing so, it has captured a glimpse of the past and it attempts a project
Immunoassay
Language: en
Pages: 613
Authors: Eleftherios P. Diamandis
Categories: Medical
Type: BOOK - Published: 1996-06-21 - Publisher: Academic Press

GET EBOOK

Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to i
Gastrointestinal Diseases and Their Associated Infections
Language: en
Pages: 285
Authors: Guy D. Eslick
Categories: Medical
Type: BOOK - Published: 2019-01-17 - Publisher: Elsevier Health Sciences

GET EBOOK

Many bacteria, viruses, protozoa, and fungi play key roles in the development of gastrointestinal diseases, and this practical reference brings you up to speed